Virus:Article Title: Pneumococcal sialidase promotes bacterial survival by fine-tuning of pneumolysin-mediated membrane disruption.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Ply Santa Cruz Biotechnology 1F11, #sc-80500; RRID: 1128666 Anti-GST FUJIFILM Wako Pure Chemical #017-21854 Anti-GFP Cell Signaling Technology D5.1, #2956; RRID: 1196615 Anti-b-Actin Santa Cruz Biotechnology C4, #sc-47778; RRID: AB_626632 Anti-SC Santa Cruz Biotechnology C-2, #sc-374343; RRID: AB_10989564 MAL ll, Biotinylated Vector Laboratories #B-1265-1 FITC-streptavidin Biolegend #405201 HRP-Goat Anti-Mouse The Jackson Laboratory RRID: AB_2313585 HRP-Goat Anti-Rabbit The Jackson Laboratory RRID: AB_2313567 Bacterial and virus strains Streptococcus pneumoniae strain R6 ATCC #ATCC BAA-255 S. pneumoniae R6/cps::nanoluc This study N/A S. pneumoniae R6/pHiBiT This study N/A S. pneumoniae R6/pHiBiT-Nanobody This study N/A S. pneumoniae R6/cps::hibit-nanobody This study N/A S. pneumoniae R6/cps::smbit-nanobody This study N/A S. pneumoniae R6 DcbpA Shizukuishi et al.16 N/A S. pneumoniae R6 DcbpA/cps::nanoluc This study N/A S. pneumoniae R6 DcbpA/pHiBiT-Nanobody This study N/A S. pneumoniae R6 DcbpA/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpA/cps::smbit-nanobody This study N/A S. pneumoniae R6 DcbpA/cps::nanoluc This study N/A S. pneumoniae R6 Dply Ogawa et al.11 N/A S. pneumoniae R6 Dply+cps::ply Ogawa et al.12 N/A S. pneumoniae R6 Dply+pSET2-ply This study N/A S. pneumoniae R6 DcbpC/cps::nanoluc This study N/A S. pneumoniae R6 DcbpD/cps::nanoluc This study N/A S. pneumoniae R6 DcbpE/cps::nanoluc This study N/A S. pneumoniae R6 DcbpF/cps::nanoluc This study N/A S. pneumoniae R6 DcbpG/cps::nanoluc This study N/A S. pneumoniae R6 DcbpL/cps::nanoluc This study N/A S. pneumoniae R6 DcbpM/cps::nanoluc This study N/A S. pneumoniae R6 DnanA/cps::nanoluc This study N/A S. pneumoniae R6 DbgaA/cps::nanoluc This study N/A S. pneumoniae R6 DstrH/cps::nanoluc This study N/A S. pneumoniae R6 DendoD/cps::nanoluc This study N/A S. pneumoniae R6 DengSP/cps::nanoluc This study N/A S. pneumoniae R6 DcbpC/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpD/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpE/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpF/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpG/cps::hibit-nanobody This study N/A S. pneumoniae R6 DcbpL/cps::hibit-nanobody This study N/A (Continued on next page) 16 Cell Reports 43, 113962, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. pneumoniae R6 DcbpM/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanA/cps::hibit-nanobody This study N/A S. pneumoniae R6 DbgaA/cps::hibit-nanobody This study N/A S. pneumoniae R6 DstrH/cps::hibit-nanobody This study N/A S. pneumoniae R6 DendoD/cps::hibit-nanobody This study N/A S. pneumoniae R6 DengSP/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanB/cps::hibit-nanobody This study N/A S. pneumoniae R6 DbgaADstrH/cps::hibit-nanobody This study N/A S. pneumoniae R6DbgaADstrHDendoD/cps::hibit-nanobody This study N/A S. pneumoniae R6 cps::PgyrAhibit-nanobody This study N/A S. pneumoniae R6 DnanA/cps:: PgyrAhibit-nanobody This study N/A S. pneumoniae R6 DnanA This study N/A S. pneumoniae R6 DnanADply/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanADply/pSET2-ply This study N/A S. pneumoniae R6 DcbpA/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanA+nanA WT/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanA+nanA DEnz/cps::hibit-nanobody This study N/A S. pneumoniae R6 DnanA+nanA WT This study N/A S. pneumoniae R6 DnanA+nanA DEnz This study N/A S. pneumoniae R6 Dply::plyAllele5 Ogawa et al. 12 N/A S. pneumoniae R6 DspxB This study N/A S. pneumoniae R6 DspxBDply Ogawa et al.11 N/A S. pneumoniae R6 DspxBDnanA This study N/A S. pneumoniae R6 DspxBDcbpA This study N/A S. pneumoniae R6 DspxBDplyDnanA This study N/A S. pneumoniae R6 DspxBDcbpADnanA This study N/A S. pneumoniae R6 DspxBDnanA+nanA WT This study N/A S. pneumoniae R6 DspxBDnanA+nanA DEnz This study N/A Streptococcus pneumoniae strain D39 NCTC #NCTC 7466 S. pneumoniae D39/pHiBiT-Nanobody This study N/A S. pneumoniae D39 Dply This study N/A S. pneumoniae D39 DspxB This study N/A S. pneumoniae D39 DspxBDply This study N/A S. pneumoniae D39 DspxBDnanA This study N/A S. pneumoniae D39 DspxBDplyDnanA This study N/A Escherichia coli strain DH10B Thermo Fisher Scientific #EC0113 Escherichia coli strain BL21 Cytiva #27154201 Escherichia coli strain NEB5a New England BioLabs #C2987 Chemicals, peptides, and recombinant proteins Cytochalasin D Cayman Chemical #22144-77-0 Heparin sodium FUJIFILM Wako Pure Chemical #085-00134 Oseltamivir phosphate FUJIFILM Wako Pure Chemical #159-03443 GFP-Trap Proteintech #gta-20 DAPI Sigma-Aldrich #D9542 Critical commercial assays Nano-Glo Luciferase Assay System Promega #N1110 LDH Cytotoxicity Assay Kit Nacalai Tesque #18250-64 (Continued on next page) Cell Reports 43, 113962, March 26, 2024 17
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